P106	The effect of resveratrol treatment on estrogen response in aorta from female and male rats.
S.Söylemez, F.Akar
Gazi University, Faculty of Pharmacy, Department of Pharmacology, Ankara, TR.

Estrogens are proposed to exert protection against cardiovascular disease. It has been shown that estrogen causes relaxation in many arteries. Resveratrol is a powerful phytoestrogen present in the skins of grapes and other plant foods and wine. Phytoestrogens are naturally occuring plant-derived nonsteroidal compounds that are functionally and structurally similar to steroidal estrogens, such as estradiol, produced by the body. In this study we aimed to investigate whether long term resveratrol treatment affect estrogen response in the artery and the difference between male and female rat Male and female rats were administered resveratrol 50 mg/L in the drinking water for 3 weeks. Dose-response curves for 17β-estradiol (10-10-10-4M) were obtained in aortic rings from both female and male rats.In endothelium intact aortic rings 17-β estradiol caused a relaxation of 76.2±3.46% (n=12) in the phenylephrine-induced tone, which was significantly enhanced to 90.96±6.37 (n=6) in resveratrol treated male rats. In female aortic rings, 17-β estradiol induced relaxation also significantly increased in resveratrol treated groups (75.42±4.44% , (n=8) vs 89.96±3.45% ,(n=7), P<0.05). In the presence of L-NOARG ,a nitric oxide synthase inhibitor, 10-4 M, estrogen-induced maximum relaxations were significantly diminished in both female and male aortic rings (76.2±3.46% vs 39.12±2.94 %(n=6); 75.42±4.44% vs 54.94±5.65 (n=6), P<0.05, respectively). Relaxation brought about 17-β estradiol is significantly inhibited by estrogen receptor antagonist 1CI 182,780 (10-6 M). These results show that estrogen produced equally relaxations in female and male aortic rings which are significantly inhibited by L-NOARG. Additionally, long term resveratrol treatment enhance the relaxing effects of 17β-estradiol in rat aortic rings.

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