Abstract EMVBM 3rd EVBM 2005

J. Vasc. Biol. 42, Sup:2 (2005) p122

P350 Inflammatory response and apoptosis induction after vascular brachytherapy: role of the peroxynitrite-poly(ADP-ribose) polymerase pathway.

¹C.J.Beller, ²E.Horvath, ¹J.Kosse, ¹L.Seres, ¹T.Radovits, ³R.Krempien, ⁴I.Berger, ¹S.Hagl, ²C.Szabó, ¹G.Szabó

¹Department of Cardiac Surgery, University of Heidelberg, Heidelberg, DE; ²Department of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, HU; ³Department of Clinical Radiology, University of Heidelberg, Heidelberg, DE; ⁴Department of Pathology, University of Heidelberg, Heidelberg, DE.

Objective: We investigated in a surgical rat model of vascular injury the potential role of the peroxynitrite - poly(ADP-ribose)polymerase pathway in inflammatory response and apoptosis induction after surgical vessel reconstruction with consecutive irradiation.
Methods: Male Sprague-Dawley rats underwent left carotid endarterectomy with removal of intima: control (n=10) or were irradiated with 15 Gray (n=13) or 20 Gray (n=10) postoperatively and compared with sham-operated rats (n=10).
Results: After 21 days, neointima formation was significantly suppressed after irradiation. However a significant inflammation of the vessel wall with focal wall necrosis was detected. Immunohistochemistry showed significant staining for nitrotyrosine, poly(ADP-ribose) and nuclear translocation of apoptosis-inducing factor in the neointima of the control group. In the irradiated groups these stainings were significantly higher in the media and adventitia compared to the non-irradiated groups.
Conclusions: Activation of the peroxynitrite - poly(ADP-ribose)polymerase pathway was demonstrated, in conjunction with the inflammatory response and apoptosis induction after surgical vessel reconstruction with consecutive irradiation. We propose that poly(ADP-ribose)polymerase may be a new target to prevent restenosis after surgical vessel reconstruction and may be able to ameliorate irradiation induced side-effects of vascular brachytherapy.

P350	Inflammatory response and apoptosis induction after vascular brachytherapy: role of the peroxynitrite-poly(ADP-ribose) polymerase pathway.
¹C.J.Beller, ²E.Horvath, ¹J.Kosse, ¹L.Seres, ¹T.Radovits, ³R.Krempien, ⁴I.Berger, ¹S.Hagl, ²C.Szabó, ¹G.Szabó
¹Department of Cardiac Surgery, University of Heidelberg, Heidelberg, DE; ²Department of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, HU; ³Department of Clinical Radiology, University of Heidelberg, Heidelberg, DE; ⁴Department of Pathology, University of Heidelberg, Heidelberg, DE.