Clin Res Cardiol (2021) DOI DOI https://doi.org/10.1007/s00392-021-01843-w

MicroRNA 183-3p is a predictor of worsening heart failure in adult patients with transposition of the great arteries and a systemic right ventricle

T. Rädle-Hurst1, N. Mackenstein1, M. Abu-Halima2, E. Meese2, H. Abdul-Khaliq1

1Klinik für Kinderkardiologie, Universitätsklinikum des Saarlandes, Homburg/Saar; 2Institut für Humangenetik, Universitätsklinikum des Saarlandes, Homburg/Saar;

Objectives: MicroRNAs (miRNAs) have been shown to play an important role in the progression of heart failure (HF). The aim of our study was to analyze miRNAs in the blood of patients with transposition of the great arteries and a systemic right ventricle (TGA-RV) in order to identify those that predict worsening HF. Methods: In 36 TGA-RV patients, SurePrint™8X60K Human v21 miRNA arrays were used to determine miRNA expression profiles and compared to age- and gender-matched controls. MiRNAs that were best related to worsening of HF (as defined by a 2-fold rise of NT-proBNP levels or the occurrence of overt HF) were further validated by RT-qPCR. Results: Using miRNA array analysis, a total of 50 down-regulated and 56 up-regulated miRNAs were found to be differentially abundant in TGA-RV patients compared to healthy controls. After validation by RT-qPCR, 5 miRNAs turned out to be significantly associated with worsening HF, namely miR-150-5p, miR-19b-3p, miR-1255b-5p, miR-423-3p and miR-183-3p. In the stepwise multivariable Cox regression analysis, ejection fraction of the systemic RV and miR-183-3p were found to be independent predictors of worsening HF (p<0.001 and p=0.008, respectively). Conclusion: In patients with TGA-RV, miR-183-3p is an independent predictor of worsening HF and thus may be used as additional biomarker in the risk assessment of these patients.

https://dgk.org/kongress_programme/jt2021/aP373.html